Impact Biologicals

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Genetic Code

		3rd nt
1st nt	2nd nt	A	G	C	T
A	A	Lys	Lys	Asn	Asn
	G	Arg	Arg	Ser	Ser
	C	Thr	Thr	Thr	Thr
	T	Ile	Met	Ile	Ile
G	A	Glu	Glu	Asp	Asp
	G	Gly	Gly	Gly	Gly
	C	Ala	Ala	Ala	Ala
	T	Val	Val	Val	Val
C	A	Gln	Gln	His	His
	G	Arg	Arg	Arg	Arg
	C	Pro	Pro	Pro	Pro
	T	Leu	Leu	Leu	Leu
T	A	***	***	Tyr	Tyr
	G	***	Trp	Cys	Cys
	C	Ser	Ser	Ser	Ser
	T	Leu	Leu	Phe	Phe

Company purpose

Biomedical researchers have a high and growing need for proteins and cell lines. Many organizations lack the time or expertise to satisfy this need. As a contract manufacturer, Impact Biologicals produces these reagents for its clients, thereby allowing clients to focus on more critical activities. Impact Biologicals provides an experienced and skilled outsourcing option for protein and cell line work. This work can include development of new methods, or routine performance / scale-up of processes that have already been developed by clients.

Philosophy

Impact Biologicals emphasizes the use of tried-and-true core technologies. It is our belief that a few key technologies, carried out rigorously using standardized procedures, provide the best opportunity to quickly produce successful results in most cases.

Core technologies

Protein expression in E. coli, typically with pET vectors
Transient transfection of mammalian cell lines to rapidly generate small amounts of processed protein for initial evaluation
Generation of stable mammalian cell lines (CHO, HEK)
Baculovirus expression in insect cells
Protein purification, often using His6 or other tags

Experience

Company founder and president Bernard Selling, Ph.D., has over twenty years of experience in the pharmaceutical industry, including seven years managing core groups responsible for protein and cell line generation at large pharmaceutical companies. He and the groups he managed have produced hundreds of proteins and cell lines for a variety of applications including antiserum production, high-throughput screening, lead optimization, and structure-based drug design employing x-ray crystallography and NMR.

Services related to proteins and cell lines

In addition to producing proteins and cell lines, Impact Biologicals carries out related services such as design and construction of expression vectors, development of ELISAs and other assays to measure protein amount or activity, development of cell-based assays, and more. Inquiries are welcome.

Consulting

Dr. Selling will visit your facility, review your procedures and results, and offer suggestions.

(610)543-6320 - info@impactbio.com - 715 Harvard Ave., Swarthmore, PA 19081

Impact Biologicals Inc.

Proteins and Cell Lines for Biomedical Research